Antioxidant Activity Test of N-Hexane, Ethyl Acetate, and Aqueous Fractions of Santang Mandarin Orange Peel (Citrus reticulata) Using the DPPH (1,1-Diphenyl-2-Picrylhydrazyl) Method
DOI:
https://doi.org/10.55927/ijsmr.v4i8.145Keywords:
Red Dragon Fruit, Antioxidant, Fractionation, Vitamin C, DPPHAbstract
This study aims to determine the antioxidant activity of the n-hexane fraction, ethyl acetate fraction, and water fraction from santang orange peel using the DPPH (1,1-Diphenyl-2-Picryl Hydrazyl) method and to determine the IC50 (Inhibitory Concentration 50) value. The Santang orange peel was extracted using the maceration method with ethanol as the solvent, followed by a multi-step fractionation process using solvents with different levels of polarity, namely the n-hexane fraction, the ethyl acetate fraction, and the water fraction. Antioxidant activity assays against DPPH radicals were conducted on the n-hexane fraction, ethyl acetate fraction, and water fraction to determine the comparative inhibitory effects of the antioxidants. The results of the study showed that the ethyl acetate fraction and the water fraction generally had higher antioxidant activity compared to the n-hexane fraction due to the presence of more polar phenolic and flavonoid compounds. The IC50 values obtained represent the concentration required to scavenge 50% of DPPH free radicals; the lower the IC50 value, the higher the antioxidant activity.
References
Ghasemzadeh, A., Jaafar, H.Z.E & Amiri, R. (2022). Antioxidant activity and bioactive compounds of Citrus nobilis peel extracts:assay. Journal of Food Science and Technology, 59 (4), 1456-1467. https://doi.org/10.1007/s13197-021-05234-8
Nurmalasari, D., Sari, R.P., & Pratama, Y. (2023). Uji aktivitas antioksidan fraksi n-heksan, etil asetat, dan air kulit buah jeruk santang (Citrus nobilis Lour. Var. microcarpa Hassk.) dengan metode DPPH. Jurnal Ilmu Farmasi Indonesia, 21(1) , 45-53. https://doi.org/10.51544/jifi.v21i1.567
Putri, A.F., Widodo, G.P., Setiawan, B., (2024). Phytochemical screening and DPPH radical scavenging activity of sewuential extracts (n-hexane, ethyl acetate,water) from jeruk peel. Indonesian Journal of Chemistry, 24(2), 210-219. https://doi.org/10.22146/ijc.78901
Rahman, F., Hidayar, T., Susanto, A., (2023). Potensi antioksidan kulit jeuk: Evaluasi fraksi n-heksan, etil asetat dan air melalui metode DPPH. Jurnal Agroteknologi, 17(2), 112-120. ISSN: 2338-4244.
Sari, N. K., Pratiwi, R., (2024). Comparative study on antiokxidant capacity of n-heksan, ethyl acetate and aqueous fractions from Citrus nobilis peel. https://doi.org/10.1016/j.focha.2023.100456
Hidayati, N., Santoso, B (2023). Aktivitas antioksidan fraksi ektrak kulit buah jeruk santang (Citrus nobilis) menggunakan metode DPPH Fraksi N-heksan, Etil Asetat dan air. Jurnal Kimia Terapan Indonesia, 11(1), 23-21. https://doi.org/10.22146/jkti.74567
Kurniawan, A., Dewi S.P., Nugroho, R.A., (2024). Isolasi dan uji antioksidan senyawa bioaktif dari fraksi n-heksan, etil asetat dan air kulit jeruk santang dengan metode DPPH. Biodiversitas Journal of Biological Diversity, 25(3), 456-465. https://doi.org/10.13057/biodiv/d250312
Mahendra, I.P., Wijaya, A. (2022). Evaluasi potensi antioksidan ekstrak dan fraksi n-heksan, etil asetat dan air kulit buah jeruk melalui scavenging DPPH. Jurnal Farmasi Galenika, 10(2), 89-98, ISSN : 2337 – 383X.
Prasetyo, D.H., Lestari , R. Fitriani, N. (2023). Phytochemical analysis and DPPH antioxidant assay of sewuential fractions from Citrus nobilis peel. Journal of Natural Medicines, 77(4), 712-721. https://doi.org/10.1007/s11418-023-01723-5
